Purification and Characterization of Two Acid Phosphatases from Germinating Peanut (Arachis hypogaea) Seed

Purification and Identification of a Natural Lectin from the Seed of Peanut Arachis hypogaea

A natural lectin from the seed of peanut Arachis hypogaea was purified by singlestep affinity chromatography using galactoside-coupled agarose. The native molecular mass of purified A. hypogaea lectin (PN-L) was 29 kDa. The lectin PN-L was detected for agglutinating activity, glycoinhibiting action and thermostability. The influence of pH on those activities was also tested. The results showed ...

Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes.

The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X₁), extraction time (X₂) and ratio of water to raw material (X₃). The optimum conditions were 85 °C, 3 h and 20:1 (m...

Characterization and purification of anthocyanins from black peanut (Arachis hypogaea L.) skin by combined column chromatography.

Black peanut skins as a byproduct from peanut industry contain abundant anthocyanins, evaluated as 8.61±0.27mg/g dry black peanut skins, are currently poorly exploited. In this work, four anthocyanins and three major flavonols were detected and identified by HPLC-PDA-ESI-MS/MS from the acidified water extract of black peanut skins of Arachis hypogaea L. After preliminary removal of flavonols by...

The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea).

Peanut agglutinin was purified by affinity chromatography on Sepharose-epsilon-aminocaproyl-beta-D-galactopyranosylamine. The purified lectin obtained in a yield of 150 mg/100 g of defatted peanut was homogeneous on polyacrylamide gel electrophoresis, ultracentrifugation, and gel filtration. This intrinsic sedimentation coefficient (So20,w) and the intrinsic diffusion coefficient (Do20,w) were ...

The Proteins of the Peanut, Arachis Hypogaea.

The ravages of the boll weevil have made it unprofitable to grow cotton in various sections throughout the South. In many regions where the growing of cotton has been decreased, peanuts are now being grown as a supplementary crop. This has caused a large increase in the production of peanuts in the United States. A number of mills which in the past produced cottonseed oil are now using their pr...

Analysis of genomic sequences from peanut (Arachis hypogaea)